课程名称︰细胞生物学概论
课程性质︰选修
课程教师︰戴桓青
开课学院：理学院
开课系所︰化学系
考试日期（年月日）︰103/06/17
考试时限（分钟）：110分钟
是否需发放奖励金：是
（如未明确表示，则不予发放）
试题 :
Intro cell Biology 2014 [Final Exam]
Answer briefly in Chinese or English, 77pts total
Q1. Membrane trafficking (12 pts)
　
　　Consider the following organelles:
(A) late endosome (B) Golgi (C) endoplasmic reticulum
(D) early endosome (E)lysosome (F) secretory vesicle
1-1. To secrete a protein such as immunoglobulin, three organelles are
involved, please list them from early to late participation. 3 pts
1-2. To endocytose a G prtein-coupled receptor for degradation, three
organelles are involved, please list them from ealy to late
participation. 3 pts
1-3. Which organelles(s) have enzymes that add cabohydrates to protein?
4 pts
1-4. Which organelle acts like "thin layer chromatography" to separate
membrane proteins and lipids? 2 pts
Q2. Cytoskeleton (15 pts)
2-1. Please match the following proteins with different types of
cytoskeleton. 6 pts
(A) kinesin; (B) beta tubulin; (C) myosin; (D) vimentin;
(E) keratin; (F) actin
(X) microfilament; (Y) intermediate filament; (Z) microtubule
2-2. From A to F, which two are motor proteins? 2 pts
2-3. Microtubules and microfilaments have a plus end and a minus end.
Which end is polymerizing more and which end is depolymerizing
more? 2 pts
2-4. Please explain the treadmilling motion of actin and the role of
ATP in treadmilling (draw a picture if necessary). 5 pts
Q3. Protein degradation (16 pts)
3-1. There are hundred of proteases in the cell. Some of them are involved
in posttranslational modification by cleaving a protein substrate.
Please explain how this type of PTM is different from pretein
degradation. 4 pts
3-2. How does the cell prevent accidental protein degradation by the
lysosome? 4 pts
3-3. Proteasome is an enzyme complex. Lysosome is an organelle. Both are
responsible for protein degradation. Which one is larger in terms
physical dimensions? 2 pts
3-4. What is ubiquitin, ubiquitination, and the function of
polyubiquitination? 6 pts
Q4. Cell signaling (17 pts)
4-1. There are many messenger molecules inside and outside the cell,
such as: (A) calcium; (B) insulin; (C) steroid hormones;
(D) IP3 (inositol trisphosphate); (E) DAG (diacylglycerol);
(F) cyclic GMP; (G) eicosanoids
Which of the above are intracellular second messengers and which
are excellular signals? 7 pts
4-2. Match Q to Z in the following picture (10 items) with the following
terms (A to J). 10 pts
(A) PIP2, phosphatidylinositol 4,5-bisphosphate
(B) IP3, inositol trisphosphate (C) diacyglycerol
(D) G protein α subunit (E) G protein β subunit
(F) G protein γ subunit (G) G prtein-coupled receptor
(H) phospolipase C (I) ligand (J) IP3 receptor
Q5. Cancer (17 pts)
5-1. Please explain why cancer is a genomic disease. 4 pts
5-2. What is the minimum number of cancer cells that is sufficient to
Cause a solid tumor? 2 pts
5-3. What is the most important tumor suppressor gene in the human genome
(often mutated in cancers)? What is its normal function? 4 pts
5-4. Please explain the relationship between proto-oncegene and oncegene.
4 pts
5-5. Please explain how cancer immunotherapy works. 3 pts
[Keep this question sheet, answer keys will be posted on CEIBA tomorrow]