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[必]工 作 量：7485英字(确认接案会请译者一起核对字数)
[必]工作报酬：15000
[必]涉及语言：英译中
[必]所属领域：生物、医学
[必]文件类型：论文(33页)、期刊(4页)
[必]截 稿 日：征到后5日
[必]应征期限：征到为止
[必]联络方式：[email protected] ; [email protected]
[必]付费方式：每月5/10/15/20/25日，汇款/转帐付清
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[选]工作要求：详见注意事项。
[选]参考段落：见试译文。
[选]试 译 文：
(2) Inhibition of fat accumulation
a. Suppresses differentiation of 3T3-L1 adipocytes (in vitro)
Experimental design and treatment:
3T3-L1 adipocytes (5x104 cells/ml) were cul
tured in DMEM medium (high glucose)
supplemented with 10% fetal calf serum. Ce
lls differentiation was induced by medium replacement containing
insulin (1 μg/ml), dexamethasone (0.25
μM) and isobutyl-methyxanthine (0.5 mM). 2 days later, the medium
containing Coffee Bean Extract and its
functional components and insulin (1μg/ml) was introduced.
The new medium was cultured for 6 days
with replacement every alternate day. Differentiation of adipocytes
and cell triglyceride levels were dete
rmined. Cell differentiation was determined using GPDH
(glycerol 3-phosphate dehydrogenase) as biochemical marker.
[选]其他事项：
1.应征者请于“同一封信”中，附上“相关学经历”及“试译文”。
2.为工作所需，文章涉及专业医学、实验及生物等专业领域，有相关背景者会优先考虑。
3.请根据撷取出之Word档进行内文翻译，会附上PDF原文参考。
3.Word档内的图片内容不需翻译。
4.征到之后会改标题，恕不一一回复，谢谢！