课程名称︰蛋白质化学与分子模拟
课程性质︰选修
课程教师︰杨健志
开课学院：生命科学院
开课系所︰生化科技学系
考试日期（年月日）︰2016/11/09
考试时限（分钟）：120分钟
试题 :
1. Please describe the principle of protein crystallization.
2. Please describe the principle of protein structure determination of x-ray
crystallography. Also, why x-ray, but not other magnetic wavelengths, is
used for atomic resolution determination of biomolecules?
3. Please describe the chemical properties of those biologically relevant metal
ions. And how these properties related to their function in biology.
4. Please describe the structure features of the major secondary structure of
proteins.
5. Please discuss the principles of protein evolution and the formation of
protein domains, based on both the chemistry and biology aspects. And
discuss how the identification of protein domains is related to the
functional studies of protein.
6. Based on the literature PS1701 provided in Ceiba, please describe at least
three protein folds in terms of their structure and function.
(For example, acetylcholine esterase fold, immunoglobulin fold, alpha/beta
hydrolase fold etc)
(编按: 该档案为Current Protocols in Protein Science (2004)
Unit 17.1: Overview of Protein Structural and Functional Folds)
7. Please discuss the principles and general steps of homology modeling of
protein.
8. Please derive the Michaelis-Menten equation for the kinetics analysis of
enzymes.
9. Please distinguish steady-state kinetics and pre-steady-state enzyme
kinetics.
10. Write a question which you ever wanted to ask in this class.
(标准杨氏风格的问题)
PS. 老师当天还叫大家多拿了张答案卷回去写，把不会写的题目查清楚后再写得更详细
(不知道是不是默认大家都会写很少)
(也不知道这怎么算分就是)