辨认切割机制蛮多种的
zinc finger、TALEN到CRISPR-Cas9都是
简单来说就是辨认序列 然后切掉他
http://www.nature.com/nprot/journal/v8/n11/full/nprot.2013.143.html
这是去年的nature protocal
有兴趣可以参考看看
动物实验已经在做了
但是要人体应用应该还很久
姑且不论该方法的问题(设计、辨认、剪切、脱钯)
这等于要把一段外源性基因插入人体
这是最大的问题所在
※ 引述《Clementtang (剑客唐唐‧光明)》之铭言：
: 美国国家科学院学报 (Early Edition) 出版前预览的最新文章
: http://www.pnas.org/content/early/2014/07/17/1405186111
: RNA-directed gene editing specifically eradicates latent and prevents new
: HIV-1 infection
: For more than three decades since the discovery of HIV-1, AIDS remains a
: major public health problem affecting greater than 35.3 million people
: worldwide. Current antiretroviral therapy has failed to eradicate HIV-1,
: partly due to the persistence of viral reservoirs. RNA-guided HIV-1 genome
: cleavage by the Cas9 technology has shown promising efficacy in disrupting
: the HIV-1 genome in latently infected cells, suppressing viral gene
: expression and replication, and immunizing uninfected cells against HIV-1
: infection. These properties may provide a viable path toward a permanent cure
: for AIDS, and provide a means to vaccinate against other pathogenic viruses.
: Given the ease and rapidity of Cas9/guide RNA development, personalized
: therapies for individual patients with HIV-1 variants can be developed
: instantly.
: 简单来说，就是透过一种可以切 DNA 的蛋白质，把 HIV 基因从被感染的细胞，
: 例如微胶细胞 (microglia) 及 T 细胞 (T Cell) 切除，并能因此避免新 HIV 感染。
: 不过目前看来困难在怎么在人体切到那些被感染的细胞，然后不会切错 XD...